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Non-human genome sequencing 

Physical
Pricing/Discount Options: Call #2
Unique Identifier: 5f9e30de-15da-47cf-a5d1-5fb60c836adc

Service Description

The Unit: Clinical Genomics Stockholm (https://www.scilifelab.se/units/clinical-genomics-stockholm/) This infrastructure is part of Science for Life Laboratory (SciLifeLab) which is an academic collaboration between Swedish universities (including Karolinska Institutet) and a national research infrastructure with a focus on life science. Clinical Genomics Stockholm unit is a research infrastructure for large-scale, genomics-based analyses using next generation sequencing technologies. The unit assists in translational research projects, in the translation of genomics-based tools to routine clinical care and also aims to improve the capability for national microbial surveillance and for pandemic preparedness.

The Service: Non-human genome sequencing Sequencing of bacterial or viral whole genomes with the possibility of sequence analysis and strain typing. Sequencing of SARS-CoV-2 amplicons. Sequencing of metagenome samples (with PCR-free protocol). This service encompasses: o Consultation (Project design, Target capture design) o Sample management o DNA& RNA sequencing (microbial genomes, metagenomics) o Bioinformatics (Bioinformatic analysis on data generated at CG) Equipment Our unit has access to specialized equipment that enable us to factilitate the translation of new high-throughput techniques into clinical use. These include, but are not restricted to: • Various automatic robotic systems (BRAVO NGS Workstation, Hamilton NGS Star) • Various systems for QC, quantification and fragment analyses (TapeStation, Quantification using fluorescent assay (Qubit, Quantit)) • Instrumentation for real time PCR and ddPCR (BioRad qPCR) • Instrumentation for Multispectral imaging (Saphyr optical mapper) • Sequencing platforms: o Illumina (NovaSeq 6000, NovaSeq 6000 Dx, NovaSeq X) o Nanopore (PromethION “access via NGI”) o PacBio (Revio)

Offerings: Research & Development Scientific & Medical Communication Testing Laboratory (bioanalysis, wet lab, *-omics, etc.)
TEF-Health Use Case Domain: all
Provider Logo Service Logo

Provider & Contact

Provider Organisation Karolinska Institutet (KI)
Provider Country Sweden
Published Email tef-health@ki.se
Billing: per project
Full Price €Sample cost: 50-300EUR/samplePersonnel cost: 115EUR/hour
Reduced Price €Sample cost: 50-300EUR/ samplePersonnel cost 0-110 EUR/h
Pricing Detail

Prices are subject to change and are indicative only. Prices vary according to numbers of samples, techniques (see below for more details) needed and according to project specifications. Consumables are included in full price cost per sample, but in some specific cases, additional reagents costs might be added. Sample price detail for each technique: o Microbial whole genome sequencing: 52EUR/sample
o SARS-CoV-2 sequencing: 48EUR/sample
o Metagenomic sequencing: 300EUR/sample

Only personnel cost (average cost of 115EUR/hour) can be subsidized up to 100% (depending upon affiliation) by TEF-Health.

Study case example of cost calculation for a whole project: o For 20 samples SARS-CoV2 seq, including a technical pilot on 4 samples and support with ethical permit application: Full price: 8 650EUR, Reduced price: 1 150EUR (subsidisation of close to 90% in this example).

Operational Details

Service Inputs Biological sample
Service Outputs Sequencing data analysis
Dependencies & Restrictions ethics vote, GDPR, Facility costs, Material Transfer Agreement
Service Standards
  • ISO 13485