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single cell RNA sequencing on CRISPR modified cells

Physical
Pricing/Discount Options: Call #2
Unique Identifier: 59a582f8-243a-4b12-b5b9-0bc2a4e632bf

Service Description

The Units: CRISPR Functional Genomics (CFG) (https://www.scilifelab.se/units/crispr-functional-genomics/) and Eukaryotic Single Cell Genomics (ESCG) (https://www.scilifelab.se/units/eukaryotic-single-cell-genomics/) Both infrastructures are part of Science for Life Laboratory (SciLifeLab) which is an academic collaboration between Swedish universities (including Karolinska Institutet) and a national research infrastructure with a focus on life science.

Both units, CFG and ESCG, have designed a pipeline of CRISPR pooled screens together with single cell RNASeq readout (Perturb-Seq, CROP-Seq). This allows to assess gene expression phenotypes at the single cell level following inactivation of a pool of genes, using Cas-9 expressing cells.

Service span the entire pooled screening process, from Cas-cell line generation and library virus creation, via phenotypic selection by FACS or live/dead, to single cell library preparation, NGS and data analysis.

Offerings: Research & Development Scientific & Medical Communication Testing Laboratory (bioanalysis, wet lab, *-omics, etc.)
TEF-Health Use Case Domain: All
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Provider & Contact

Provider Organisation Karolinska Institutet (KI)
Provider Country Sweden
Published Email tef-health@ki.se
Billing: per project
Full Price €From 1000EUR per projectPersonnel cost: 115EUR/hour
Reduced Price €From 1000EUR per projectPersonnel cost 0-110 EUR/h
Pricing Detail

Prices are subject to change and are indicative only. The final price will be determined by a quote based on SME's needs and the project requirements, and it will be validated by the contract between the service provider and the applicant. Only personnel cost (average cost of 115EUR/hour) can be subsidized up to 100% (depending upon affiliation) by TEF-Health. Consumables will be charged at full price cost.

Operational Details

Service Inputs Biological sample
Service Outputs Sequencing data analysis
Dependencies & Restrictions ethics vote, GDPR, Facility costs, Material Transfer Agreement